Dual-wavelength spectrophotometry in compound Dyclonine Dyclonine Paint and sali

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Dual-wavelength spectrophotometry in compound Dyclonine Paint and salicylic acid Dyclonine
 
 
Key words] R927.2; R977 [Document code] A [Article ID] 1004-0781 (2004) 12-0947-02DeterminationoftheContentofInosineinInosineGlucoseInjectionwithHPLCUYu. zhen, XUYu. hong, CHENxia0. kai, LICheng, LUWei (DepartmentofPharmacy, ianPeople'SttospitaZoftheCityofShenzhen. Shenzhen518033.China) MlffrRACTObjective: TosetupanI-IPIA ~ methodforthedeterminationofthecontentofinosineintheinosineglucoseinjection. Methods: AHypersilODSCI8columnwasemployed. Themobilephaseconsistedofmethanolandwater (10:90), withaflowrateof1.0mL · min edetectionwavelengthwas248nn1. Results: AgoodlinearrelationshipWasshownwhentheconcentrationsofimositolWasintherangeof8-40Pg · mL-'(r = 0.9999). rIaveragerecoveryratewas102.3%, RSD = 0.7% (n = 5). Condusion: Themethodwasshowntobehandy, quickandaccurateinthedetermination. KEYWORDSlnosine; HIighperformanceliquidchromatography (PIC); Determination of inosine can participate in energy metabolism and protein synthesis in vivo, to improve the body metabolism. Poor thermal stability of inosine on the production, storage or external conditions change prone to break down under ..., decomposition products of the UV absorption peak of hypoxanthine and inosine UV absorption peak overlap, and the absorbance higher than the absorbance of inosine Therefore, measured by ultraviolet spectrophotometry of creatinine levels often high J, high performance liquid chromatography author Inosine Inosine injection of glucose content, with satisfactory results. 1 Apparatus and reagents Agilent1100 performance liquid chromatography (Anjie Lun U.S. companies), VWD UV detector, Agilent ChemStation, UV. 2201 UV spectrophotometer (Shimadzu); inosine reference substance (purity 99.9%, [Received Et period] 2003-11-06 [Revised] 2003-12-25 [Author] Li Yuzhen (1970 a), F, Shaoguan, officer pharmacists, bachelor, in the hospital pharmacy. Shenzhen Institute for Drug Control to provide), inosine and glucose injection (specification 0.6g: 100mL,You are not allowed to view links. Register or Login, lot number: 0305044,0305173,03060522, Jiangsu Chen Brand Pharmaceutical Co., Ltd.); methanol (HPLC grade,You are not allowed to view links. Register or Login, Tianjin, China West Special Reagent Factory). 2 Methods and Results 2.1 Chromatographic conditions Column: HypersilODSCl8 (Agilent5/an4.0mill × 125.0mm), mobile phase: methanol. Water (10:90), flow rate: 1.0mL · min, detection wavelength: 248nln, the column temperature was at room temperature, injection volume: 20,You are not allowed to view links. Register or Login, sensitivity 0.005AUFS. Under these conditions, theoretical plate number calculated by peak creatinine ≥ 2000, the test separation from other materials not less than 1.5, the peak area of ​​external standard method. 2.2.1 Solution Preparation 2.2 Preparation of standard solutions of inosine reference substance, accurately weighed about 200mg, home 100mL flask,You are not allowed to view links. Register or Login, diluted to the mark with the mobile phase after shaking set precise amount of 10mL 100mL flask,You are not allowed to view links. Register or Login, add the mobile phase was diluted to