Muscle injury and gene therapy vector pIRES2-EGFP-MyoD Construction and expressi

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Muscle injury and gene therapy vector pIRES2-EGFP-MyoD Construction and expression in MSCs
 
 
Guarantee a certain extent,You are not allowed to view links. Register or Login, stable gene transfer ; (2 ) contains IRES, can enhance the level of translation of target gene expression ; (3 ) containing kan,You are not allowed to view links. Register or Login, neu gene , the former can be screened sulfate kalamycin prokaryotic cells ; the latter can be G418 To filter the eukaryotic target cells. In summary, our experiments using molecular cloning technology, the restriction that they have successfully built pIRES2 of a EGFP-MyoD eukaryotic expression vector , and this plasmid was transfected into purified M_SCs in ,You are not allowed to view links. Register or Login, and for a positive transfection Cloning and transfer cell culture, I believe that with the deepening of the study , for further study in the subtle muscle damage ,You are not allowed to view links. Register or Login, muscle tumors of the biological role and mechanism to provide direct experimental evidence , but also for its gene Therapy of 【 9J.