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- March 09, 2011, 09:20:11 AMDietary calcium in myocardial necrosis of Keshan disease in the role: the isolated rat heart ...
189mmHg, PCO: 13. ] MmHg, pH7.4. 1.2 Lmagendorff simple method of low calcium perfused rat hearts before filling with 1.0mmol/LfreeCaH displaced the pre-K-H solution irrigation 15rain, stabilize and then shaking with different freeCa the K-H perfusion 60rain, CK observed in the amount of effluent. 1.5 calcium combined hypoxic perfused for the Eighth Five-Year Project of the topic, from Bethune Medical University, completed a 258 - Langendorff isolated heart method pre-irrigation irrigation displaced after 15rain, 0.6,You are not allowed to view links. Register or Login, O. 5, O. 4retool / L calcium in each group filling the first 10min, then calcium, hypoxia irrigation 15rain, CK Determination of the amount of flow crossing the control group in hypoxia] Smin. 1.4 out of mechanical CK was determined by the Beijing high-tech biological engineering students provided kit. M750 A A utility meter UV-Vis spectrophotometer stag,You are not allowed to view links. Register or Login, wavelength 340T1m,You are not allowed to view links. Register or Login, water bath at 30C. 2 Results 2.1 inotropic calcium alone perfused, freeCa 1. O ~ O. 6mmol / L during the period, the total calcium in the 2,5 retool ~】. 5mmol / L between the no effect on myocardial contraction; when less than O. 6mmol / L or less, myocardial contractility was significantly decreased (see Table 1). Decline a canal http fluid total calcium (Ca L996 Volume 11 No. 5 in the simple low calcium experiments,You are not allowed to view links. Register or Login, the amount of effluent CK decreased with the increase freeCa +. was a significant negative correlation (see Table 2) Table 2 in low calcium perfusion effluent CK ■ (U / L, ±.) computer integrated analysis of the significant differences between groups in the low calcium, low oxygen perfusion experiment, when calcium hypoxia perfusion,You are not allowed to view links. Register or Login, the flow significantly, the difference was significant (Table 3). yet another increase in the amount of fluid in the CK , the low calcium group than hypoxic hypoxia calcium calcium combined hypoxia in table 5 in the effluent CK ■ (U / L, i ±.) a.c-d, P <0-015 d of calcium as a discussion of life elements, involved in various important physiological activities. extracellular calcium to maintain normal cardiac structure and contractile cells of myocardial enzyme release and cell membrane damage appears irreversible damage to the sign]. In this study, dietary calcium and myocardial necrosis of Keshan disease This view plays an important role in obtaining evidence, using in vitro experiments to observe the relationship between calcium and myocardial injury. The results show that when the perfusate freeCa Leakage of intracellular enzymes increased, effluent CK increase. When freeCa one in 0.6retooll, L below. Significantly decreased myocardial contractility and enzyme flow increased significantly. Then accompanied by hypoxia, the cell leakage of CK into a 'step increase. Calcium hypoxic hypoxia group was significantly higher than that of calcium. P <0.0l, tips calcium combined hypoxia on heart damage from the camera body 5, a great research shows that when the whole animal blood freeCa less than 0.5mmol / L. Calcium (Ca) in 1.0rnmol / L lower, the combination of factors Guo oxygen can cause significant myocardial necrosis 0】. The results support the low calcium can cause myocardial damage theory.
189mmHg, PCO: 13. ] MmHg, pH7.4. 1.2 Lmagendorff simple method of low calcium perfused rat hearts before filling with 1.0mmol/LfreeCaH displaced the pre-K-H solution irrigation 15rain, stabilize and then shaking with different freeCa the K-H perfusion 60rain, CK observed in the amount of effluent. 1.5 calcium combined hypoxic perfused for the Eighth Five-Year Project of the topic, from Bethune Medical University, completed a 258 - Langendorff isolated heart method pre-irrigation irrigation displaced after 15rain, 0.6,You are not allowed to view links. Register or Login, O. 5, O. 4retool / L calcium in each group filling the first 10min, then calcium, hypoxia irrigation 15rain, CK Determination of the amount of flow crossing the control group in hypoxia] Smin. 1.4 out of mechanical CK was determined by the Beijing high-tech biological engineering students provided kit. M750 A A utility meter UV-Vis spectrophotometer stag,You are not allowed to view links. Register or Login, wavelength 340T1m,You are not allowed to view links. Register or Login, water bath at 30C. 2 Results 2.1 inotropic calcium alone perfused, freeCa 1. O ~ O. 6mmol / L during the period, the total calcium in the 2,5 retool ~】. 5mmol / L between the no effect on myocardial contraction; when less than O. 6mmol / L or less, myocardial contractility was significantly decreased (see Table 1). Decline a canal http fluid total calcium (Ca L996 Volume 11 No. 5 in the simple low calcium experiments,You are not allowed to view links. Register or Login, the amount of effluent CK decreased with the increase freeCa +. was a significant negative correlation (see Table 2) Table 2 in low calcium perfusion effluent CK ■ (U / L, ±.) computer integrated analysis of the significant differences between groups in the low calcium, low oxygen perfusion experiment, when calcium hypoxia perfusion,You are not allowed to view links. Register or Login, the flow significantly, the difference was significant (Table 3). yet another increase in the amount of fluid in the CK , the low calcium group than hypoxic hypoxia calcium calcium combined hypoxia in table 5 in the effluent CK ■ (U / L, i ±.) a.c-d, P <0-015 d of calcium as a discussion of life elements, involved in various important physiological activities. extracellular calcium to maintain normal cardiac structure and contractile cells of myocardial enzyme release and cell membrane damage appears irreversible damage to the sign]. In this study, dietary calcium and myocardial necrosis of Keshan disease This view plays an important role in obtaining evidence, using in vitro experiments to observe the relationship between calcium and myocardial injury. The results show that when the perfusate freeCa Leakage of intracellular enzymes increased, effluent CK increase. When freeCa one in 0.6retooll, L below. Significantly decreased myocardial contractility and enzyme flow increased significantly. Then accompanied by hypoxia, the cell leakage of CK into a 'step increase. Calcium hypoxic hypoxia group was significantly higher than that of calcium. P <0.0l, tips calcium combined hypoxia on heart damage from the camera body 5, a great research shows that when the whole animal blood freeCa less than 0.5mmol / L. Calcium (Ca) in 1.0rnmol / L lower, the combination of factors Guo oxygen can cause significant myocardial necrosis 0】. The results support the low calcium can cause myocardial damage theory.