Suppression subtractive hybridization glycyrrhizin rat hepatic stellate cells down the target gene
, QIUXiang-jun, CHENXing-yan, JIANGWen-gao. , HUGuo-xin (1.DepartmentofPharmacology, CollegeofPharmacy, WenzhouMedicalCollege,belstaff milano (http://www.belstaffmilano.net), Wenzhou325027, China; 2.DepartmentofPharmacology, MedicalCollegeofttenanUniversityofScienceandTechnology, Luoyang471003, China) ABsTltACTObjectiveAHPLCmethodwasestablishedforthedeterminationofcarbamazepine (CBZ) and10. 11-epoxidecarbamazepine (ECBZ) inplasmaandcerebrospinalfluid, andtoinvestigateitspharmaeokinetiecharacteristicsinrabbits. MethodsWithoxcarbazepinea8theinternalstandard. CBZandECBZweredetectdedbyUVdetectorat210nmwiththeHypersil-ODSC18columnandacetonitrile-water (63:37) asthemobilephaseataflowrateof0.8mL · min ~. Thedataobtainedweretreatedwith3P87pharmacokineticprogramoncomputer. ResultsThelinearrangeWaS0.05 a lO. 00mg · L '(r = 0,9999). Themeanrecoveriesoftheassaywere99.6% a 105.4%. Boththeintra-dayandinter-dayPrecisions (RSD) werelessthan7%. ConclusionThismethodissimple. sensitiveandaccurate, ItcallbeusedforthedetectionandpharmacokineticstudiesofCBZandECBZinrabbits. KEYWORDSCarbamazepine; HPLC; Pharmacokinetic carbamazepine (carbamazepine,belstaff outlet (http://www.belstaffoutlets.org), CBZ) is commonly used in clinical dysentery anti-epilepsy drugs,p90x calendar (http://www.p90xworkoutscalendar.com), due to relatively narrow therapeutic window, efficacy and toxicity and blood drug 【Received Date] 2005-03-14 【Fund] Wenzhou Science and Technology Bureau issues (Fund Code: Y2003A10) [Author] Zhou Hongyu (1966), female, Zhejiang Wenzhou, Associate Professor, Master. Mainly engaged in Neuropharmacology and Pharmacokinetics. Tel :0577-86689710, E-mail: lxd54 @ 163. corn. [Correspondence] Guo-Xin Hu (1957 a), male, professor, mainly engaged in clinical pharmacology. Tel :0577 -86689710. Closely related to the concentration, the current detection method and on its pharmacokinetics study more, but in terms of pharmacokinetics generally describes the process of blood concentration of CBZ ¨ J,timberland portugal (http://www.timberlandshoesportugal.com), and its metabolites in the cerebrospinal fluid dynamic process I have not been reported in . The results show that, CBZ major metabolite in the body 1O,tory burch outlet (http://www.toryburchshoesoutlet.org), 1l an epoxide carbamazepine (10,11-epoxidecarbamazepine, ECBZ) has a similar anti-epileptic activity with CBZ, and CBZ occurred after treatment of a number of adverse events, and CBZ In the long-term use or in combination with other drugs CBZ and ECBZ body will change the proportion of population J, so understanding the cerebrospinal fluid in the body, especially the dynamic over ECBZ